平铺镇农村人居环境整治研究与探讨

通过对繁昌县平铺镇农村人居环境整治情况的调查,发现存在整治难、维护难等一系列问题,对其成因进行了分析,并提出了相应的应对措施。     

Introduction to the International Symposium on Lipid Science and Health and research progress in lipid science and health

The International Symposium on Lipid Science and Health (ISLSH), which was organized annually by Oil Crops Research Institute of Chinese Academy of Agricultural Sciences (OCRI-CAAS) since 2016, has gained a strong reputation and attracted hundreds of delegates from around the world for discussion of lipid research trends and advances every year, to promote research and academic exchanges in the fields of lipid science and health. The 5th International Symposium on lipid Science and Health was successfully held in Wuhan, China, from October 23rd to 25th, 2020, to celebrate the 60th anniversary of OCRI-CAAS. The two-day symposium gathered well-known experts specialized in lipid science to share the current state of lipid research with emphasis on aspects covering: (1) lipid profiling and characterization, (2) lipid preparation and modification, (3) lipid improvement and regulation, and (4) lipid nutrition and health. The symposium was conducted by a combination of on-site and network meeting. More than 250 distinguished delegates from academia and industry participated in the on-site multidisciplinary meeting, and thousands of scholars attended the virtual event. This paper is as a record of the symposium proceedings and a brief summary of the advances and trends in 4 aspects of lipid science and health.     

Zinc biofortification of maize (Zea mays L.): Status and challenges

Zn deficiency is one of the leading health problems in children and women of developing countries. Different interventions could be used to overcome malnutrition, but biofortification is most impactful, convenient, sustainable and acceptable intervention. Maize is one of the major crops grown and consumed in the regions with prevalent Zn malnutrition; therefore, this is suitable target for Zn biofortification. Zn biofortification of maize could be achieved through agronomic and genetic approaches. Discussion of agronomic approaches with genetic approaches is prerequisite because soils in developing countries are deficit of Zn and availability of Zn in soils is mandatory for estimating the genetic responses of maize genotypes through genetic approaches. Seed priming, foliar and soil applications are agronomic tools for biofortification, but solo and combined applications of these treatments have different effects on Zn enrichment. Genetic approaches include the increase of Zn bioavailability or increase of kernel Zn concentration. Zn bioavailability could be increased by reducing the anti‐nutritional factors or by increasing the bioavailability enhancers. Kernel Zn concentration could be improved through hybridization and selections, whereas genetically engineered attempts for improving Zn uptake from soil, loading in xylem, remobilization in grains and sequestration in endosperm can further improve the kernel Zn concentration. Key challenges associated with dissemination of Zn biofortified maize are also under discussion in this draft. Current review emphasized all of above‐mentioned contents to provide roadmap for the development of Zn biofortified maize genotypes to curb the global Zn malnutrition.     

秸秆或粉煤灰添加对砂姜黑土持水性及小麦抗干旱胁迫的影响

针对黄淮海平原典型中低产土壤砂姜黑土黏粒含量较高,土壤有效水分库容较低,严重限制作物生产的现状,该文采用盆栽试验,研究了不同外源改性物料的添加对土壤持水性能及小麦生理的影响,以期获得农田水分管理过程中的关键参数。盆栽试验设置常规氮磷钾(control,CK),常规氮磷钾配施下的添加秸秆(straw returning,SR)、秸秆碳(straw carbon,SC)和粉煤灰(fly ash,FA)处理,维持土壤相对含水率在80%,培育小麦至抽穗期,开展为期10 d的干旱胁迫试验。结果显示SR和SC处理提高了土壤持水能力,且处理间的差异较小;FA处理因其表面富含大量疏水性结晶矿物,土壤相对含水率下降较快,迟效水含量显著低于其余处理,但土壤速效水含量显著提高。不同改性措施均有提高小麦叶片相对含水率,减轻干旱胁迫的趋势,但在极端干旱胁迫下,FA处理叶片相对含水率不仅明显低于其余处理,且作物体内积累大量丙二醛、过氧化氢等有害物质。田间管理中砂姜黑土相对含水率应维持在38%(SR)、36.5%(SC)和24.5%(FA)以上,当土壤相对含水率低于30.78%(SC)、28.43%(SR)和22.5%(FA)时将会对作物生理产生不可逆的伤害。鉴于秸秆优良的保水性能,粉煤灰“富水,不保水”的特性,秸秆与粉煤灰的配合施用将利于砂姜黑土的改良。相关机制值得进一步研究。     

Variation in glomalin in soil profiles and its association with climatic conditions,shelterbelt characteristics,and soil properties in poplar shelterbelts of Northeast China

Glomalin-related soil protein(GRSP)sequesters large amounts of carbon and plays important roles in maintaining terrestrial soil ecosystem functions and ecological restoration;however,little is known about GRSP variation in 1-m soil profiles and its association with stand characteristics,soil properties,and climatic conditions,hindering GRSP-related degraded soil improvement and GRSP evaluation.In this study,we sampled soils from 1-m profiles from poplar(Populus spp.)shelterbelts in Northeast China.GRSP contents were 1.8–2.0 times higher in the upper 40 cm soil layers than at 40–100 cm.GRSP-related soil organic carbon(SOC)sequestration in deeper soil layers was*1.2 times higher than in surface layers.The amounts of GRSP-related nutrients were similar throughout the soil profile.A redundancy analysis showed that in both surface and deeper layers,soil properties(pH,electrical conductivity,water,SOC,and soil nutrients)explained the majority of the GRSP variation(59.5–84.2%);the second-most-important factor in GRSP regulation was climatic conditions(temperature,precipitation,and altitude),while specific shelterbelt characteristics had negligible effects(<5%).Soil depth and climate indirectly affected GRSP features via soil properties,as manifested by structural equation model analysis.Our findings demonstrate that GRSP is important for carbon storage in deep soils,regardless of shelterbelt characteristics.Future glomalin assessments should consider these vertical patterns and possible regulating mechanisms that are related to soil properties and climatic changes.     

卷丹转基因体系构建及岷江百合LrCCoAOMT的导入

以卷丹（Lilium lancifolium Thunb.）无菌小鳞片为试材，通过切片处理、优化农杆菌侵染浓度与时间以及重悬液和共培养基成分，构建农杆菌介导的高效遗传转化体系。结果表明，MS + 1.5 mg ? L-1 6-BA + 0.5 mg ? L-1 NAA + 30 g ? L-1蔗糖是切片芽分化的最佳培养基，添加100 mg ? L-1抗坏血酸能有效抑制褐化并促进不定芽增殖。MS + 2.0 mg ? L-1 NAA + 30 g ? L-1蔗糖是生根诱导的最佳培养基。抗生素敏感性测试发现，培养基中添加Kan 100 mg ? L-1或Hyg 75 mg ? L-1 结合Cef 400 mg ? L-1适宜抗性筛选。GUS染色分析表明，以去除大量元素的改良MS + 100 μmol ? L-1 AS和去除大量元素的改良MS + 1.0 mg ? L-1 6-BA + 1.0 mg ? L-1 NAA + 100 μmol ? L-1 AS为重悬液和共培养基，将切片在农杆菌菌液浓度OD600为0.4，侵染15 min，获得81.72% 瞬时转化率和25.2% 稳定遗传转化率。将岷江百合LrCCoAOMT转化卷丹，分子检测和GUS染色分析表明已获得转基因阳性株系。     

Embryo-mediated genome editing for accelerated genetic improvement of livestock

Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.     

在宽泛的稠度范围内估算面粉标准粉质参数的可行性探索

为在宽泛的稠度范围内检测和估算小麦的粉质参数以服务品质育种，本研究通过构建和应用复合粉质参数，并结合梯度加水、目测加水和误差控制技术，明显降低了加水量对测定结果的影响，成功建立了一系列逻辑算式和回归方程，定量描述了宽泛稠度与标准稠度（500 FU）下检测结果之间的转换关系，实现了标准检测结果的非直接测定。目测加水估计结果与对应标准值的相关性显示，形成时间、稳定时间、粉质质量指数和弱化度的估计标准值与标准值间的相关系数r=0.988～0.995；从不同途径推导出标准加水量预测的三个算式，其预测结果与标准值的相关系数r=0.983～0.995，综合预测效果明显优于单纯粉质仪内置软件的预测值。从而，用小而确定的样品量与一次目测加水测定（推荐稠度440～650 FU）估计标准检测结果，且可据此准确地调整加水量，直接测得达标的粉质参数。另外，借用逻辑算式还可将全部参数的达标值矫正为稠度500 FU时的标准值，使传统的粉质检测结果更加精准、更具可比性。     

西藏地区青稞籽粒营养品质分析及评价

为探明西藏地区青稞品种间的性状差异及性状间的相关性，筛选优异品质青稞种质，以251个西藏青稞种质资源为材料，测定了籽粒淀粉、粗蛋白、β-葡聚糖、γ-氨基丁酸、大量元素（Ca、P）、微量元素（Fe、Zn、Se）含量共9个品质指标，分析了被测指标间关系。结果表明，所测定的9项品质指标在供试种质资源间均存在不同程度的差异，淀粉含量的变异系数最小，Se含量的变异系数最大；粗蛋白与淀粉含量呈极显著负相关（r= -0.54），粗蛋白与γ-氨基丁酸、Ca、P、Zn、Fe含量均呈极显著正相关。对被测指标进行主成分分析发现，可将9项品质指标简化成3个主成分，其累积方差贡献率为74.5%，其中第一主成分因子的贡献率达到了 48.0%，第一主成分中因子载荷量大的品质指标是Zn、P、Ca、粗蛋白、γ-氨基丁酸、淀粉、Fe含量。综合评价筛选出10个营养品质较高的种质，分别为BJX152、BJX008、BJX149、BJX004、BJX003、BJX015、BJX005、藏青2000、BJX227、喜马拉22号。